ION Biosciences’ Brilliant Intracellular pH Assay is a high-throughput, no-wash, fluorescence-based solution optimized to monitor activation of plasma membrane proton-coupled channels and transporters in response to pharmacological compounds. These include the voltage-gated proton channel (HV1), Na⁺/H⁺ exchangers (SLC9), divalent metal transporters (SLC11), peptide transporters (SLC15), monocarboxylate transporters (SLC16), proton-coupled amino acid transporters (SLC36), proton-coupled sugar transporters (SLC45), and the proton-coupled folate transporter (SLC46). Kit contains enough reagents to conduct 10 plate-based assays.